2012年12月17日 星期一

【influenza H1N1流感HA蛋白質】貨號#11085-V08H。Influenza H1N1 ( A/California/7/2009 ) Hemagglutinin HA 蛋白質

【產品名稱】

Influenza H1N1 ( A/California/7/2009 ) Hemagglutinin HA 蛋白質 Product Information


Synonym:HA
Protein Construction:A DNA sequence encoding the extracellular domain of Influenza A virus H1N1 ( A/California/07/2009 ) ( ACP44189.1 ) hemagglutinin ( Met 1 - Gln 529 ) ( HA1+HA2, two amino acids deletion, uncleaved ) was fused with a polyhistidine tag at the C-terminus
Source:Influenza A Virus H1N1
Expression Host: Human Cells

【產品規格】 200ug、500ug、1mg

【產品特點】

H1N1 ( A/California/7/2009 ) HA Protein QC Testing

Purity:> 97 % as determined by SDS-PAGESDS-PAGE:

H1N1 HA protein

Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:Asp 18
Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus H1N1 ( A/California/07/2009 ) comprises 521 amino acids with the predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions
Formulation:Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

【產品保存方法】
H1N1 ( A/California/7/2009 ) HA Protein Usage Guide

Storage:Store it under sterile conditions at -70℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

【相關流感研究試劑】

H1N1 ( A/California/7/2009 ) HA Related Influenza Virus Research Tools


【產品Datasheet】11085-V08H
H1N1 HA Protein Description

Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.


【文獻參考】
References

  1. Günther-Ausborn, S. et al., 2000, J. Virol. 74: 2714-2720.
  2. Hoffman, LR. et al., 1997, J. Virol. 71: 8808-8820.
  3. Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-16715.
  4. Gething, M-J. et al., 1980, Nature. 287: 301-306.
  5. Naeve, C.W. and Williams, D. 1990, EMBO. J. 9: 3857-3866.

【相關流感研究試劑】

Influenza Hemagglutinin (HA) Protein Products

Influenza Hemagglutinin (HA) Protein: H1N1, A/California/07/2009

Product (CLICK for detailed Info. and price)SpeciesMoleculeDescriptionCat No
Influenza Hemagglutinin (HA) Protein
H1N1, A/California/07/2009		Influenza A H1N1 VirusHAHis Tag11085-V08H

【產品訂購資訊】

【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

【原廠網址】http://www.sinobiological.com


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