【產品名稱】
Influenza A H1N1 Nucleoprotein / NP Protein【產品規格】
· 200 μg:
· ≥1mg Bulk:
【產品特點】
H1N1 NP / Nucleoprotein Protein Product Information
Synonym :
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NP
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Protein Construction:
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A DNA sequence encoding the Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein (B4URE0.1) (Met 1-Gly 490) was fused with a polyhistidine tag at the C-terminus.
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Source:
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Influenza A Virus H1N1
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Expression Host:
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Baculovirus-Insect cells
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H1N1 NP / Nucleoprotein Protein QC Testing
Purity:
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> 97 % as determined by SDS-PAGE
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SDS-PAGE:
H1N1 NP protein
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Endotoxin:
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< 1.0 EU per μg of the protein as determined by the LAL method
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Stability:
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Samples are stable for up to twelve months from date of receipt at -70℃
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Predicted N terminal:
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Met 1
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Molecular Mass:
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The recombinant Influenza A virus ( A/Puerto Rico/8/34/Mount Sinai (H1N1) ) nucleoprotein comprises 501 amino acids and has a predicted molecular mass of 56.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions
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Formulation:
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Lyophilized from sterile 60mM Tris, 500mM NaCl , pH 7.4
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【產品保存方法】
H1N1 NP / Nucleoprotein Protein Usage Guide
Storage:
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Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Reconstitution:
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A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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【產品Datasheet】
11675-V08B
H1N1 NP / Nucleoprotein Protein Description
Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species. Influenza B is largely confined to humans and is an important cause of morbidity. Influenza C infects humans, dogs and pigs, sometimes causing both severe illness and local epidemics. Influenza A is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). The notation HhNn is used to refer to the subtype comprising the hth discovered HA protein and the nth discovered NA protein. Influenza viruses A, B and C are very similar in overall structure. The viral particles of all influenza viruses are similar in composition. These are made of a viral envelope containing two main types of glycoproteins, wrapped around a central core. The central core contains the viral RNA genome and other viral proteins that package and protect this RNA. The influenza A genome contains 11 genes on eight pieces of RNA, encoding for 11 proteins: Hemagglutinin (HA), Neuraminidase (NA), Nucleoprotein (NP), M1, M2, NS1, NS2(NEP), PA, PB1, PB1-F2 and PB2.
Influenza A virus nucleoprotein (NP) forms homo-oligomers and multiple copies of NP wrap around genomic RNA, along with a trimeric polymerase making up ribonucleoprotein (RNP) complex. NP is composed of a head and a body domain and a tail loop / linker region. The head domain is more conserved than the body domain. NP oligomerization is mediated by the insertion of the non-polymorphic and structurally conserved tail loop of one NP molecule to a groove of another NP. The different form of NP oligomers is due to the flexibility of the polymorphic linkers that join the tail loop to the rest of the protein. The RNA binding property of NP is known to involve the protruding element and the flexible basic loop between the head and body domains, both having high degree of primary sequence conservation.
Influenza A virus nucleoprotein (NP) forms homo-oligomers and multiple copies of NP wrap around genomic RNA, along with a trimeric polymerase making up ribonucleoprotein (RNP) complex. NP is composed of a head and a body domain and a tail loop / linker region. The head domain is more conserved than the body domain. NP oligomerization is mediated by the insertion of the non-polymorphic and structurally conserved tail loop of one NP molecule to a groove of another NP. The different form of NP oligomers is due to the flexibility of the polymorphic linkers that join the tail loop to the rest of the protein. The RNA binding property of NP is known to involve the protruding element and the flexible basic loop between the head and body domains, both having high degree of primary sequence conservation.
【相關流感研究試劑】
Related Influenza Virus Research Tools
【文獻參考】
References
1. Li KS, et al., 2004, Nature. 430: 209-13.
Epstein, S.L. et al., 2005, Vaccine. 23 (46-47): 5404-10.Christophe F, et al., 2009, Science. 324: 1557-61.
Guan Y, et al., 2009, Revue scientifique et technique. 23(2): 453-65.
Ng, A.K. et al., 2009, Sci China C Life Sci. 52 (5):439-49.
【產品訂購資訊】
【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
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