重組蛋白產品抗體純化單多株抗體客製服務: GFP多株抗體。WB應用、IF應用。萊恩生物科技有限公司代理BIOVISION

2012年12月19日星期三

GFP多株抗體。WB應用、IF應用。萊恩生物科技有限公司代理BIOVISION

GFP 多株抗體Polyclonal Antibody (100 ug)

Creighton J et al (2008) J. Cell Sci. 121: 110 - 119.

HOST: Rabbit
IMMUNOGEN: Recombinant EGFP (Internal ID# BV-315) SPECIES: All
FORMULATION: 100 ug (0.2 mg/ml) affinity purified rabbit anti-GFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
BACKGROUND: Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
SPECIFICITY: The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP (Cat.# 4999-100), RFP (Cat.# 4997-100), YFP (Cat.# 4998-100) and CFP (Cat.# 4996-100) can be used as positive controls.
APPLICATION AND USAGE: The antibody can be used for Western blotting (0.5-4 ug/ml). Based on researcher's feedback, it can also be used in immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually.

STORAGE CONDITIONS: Store at -20oC. For long-term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.